Hybeacon probes
Web10 dec. 2010 · Six-colour HyBeacon probes for multiplex genetic analysis Chembiochem. 2010 Dec 10;11(18):2530-3.doi: 10.1002/cbic.201000623. Authors James A Richardson 1 , Marta Gerowska, Montserrat Shelbourne, David French, Tom Brown Affiliation 1School of Chemistry, University of Southampton, Southampton SO17 1BJ, UK. Web1 dec. 2024 · Our work details the development of three HyBeacon® probes, used for the presumptive detection of rhinoceros, pangolin and key target species in the Panthera genus. The approach can be performed in a single tube using melt curve analysis and provide rapid assessment of sample identity.
Hybeacon probes
Did you know?
WebSynthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis - Chemical Communications (RSC Publishing) Issue 11, 2003 Previous Article Next Article From the journal: Chemical Communications Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis † Web1 dec. 2001 · Hybridization of HyBeacons to complementary DNA target sequences results in a measurable elevation of probe fluorescence emission. HyBeacon probes may be incorporated into real-time polymerase chain reaction (PCR) assays to detect the presence and monitor the accumulation of specific DNA sequences.
Web1 apr. 2024 · The specificity of a HyBeacon probe to its complementary sequence and its detection using melt curve analysis may solve many of the processing issues currently … WebHyBeacon probes are single-stranded oligonucleotides with one or more internal base(s) labeled with a fluorescent dye. When a probe forms a duplex with its target sequence, the level of ...
Web14 mei 2024 · These HyBeacon probes consist of an oligonucleotide sequence specific for the STR target, one or several internal FAM fluorophore dT-analogues and a 3′ phosphate cap to prevent PCR extension. Web2 jun. 2010 · HyBeacon probes have been used to characterise SNPs in the CYP2C9 and VKORC1 genes associated with variations in the efficiency of warfarin metabolism. PCR …
WebA combination of sixHyBeacon probes has been used in multiplex genetic analysis to analyse mutations in the CFTR gene of PCR-amplified human DNA. Multicolour …
Web1 dec. 2001 · Hybridization of HyBeacons to complementary DNA target sequences results in a measurable elevation of probe fluorescence emission. HyBeacon probes may be incorporated into real-time polymerase chain reaction (PCR) assays to detect the presence and monitor the accumulation of specific DNA sequences. high end custom golf cartsWebHyBeacon probes are included in homogeneous PCR assays to genotype samples with respect to the factor V polymorphism within 20 min, using purified DNAs and unpurified … high end cutlery brandsWeb8 jan. 2024 · HyBeacon probes can be integrated into real-time PCR analysis to detect the presence and monitor the accumulation of specific DNA targets. In addition, sequence differences as small as a single nucleotide can be detected and differentiated by measuring the melting temperature. high end custom riflesWebThe work presented here details the development of a single-step one-tube RT-PCR assay to detect the presence of body fluids of forensic interest (saliva, blood, seminal fluid, … high end cutlery setsWeb3 aug. 2007 · HyBeacon probes The probes employed for the analysis of PRNP polymorphisms were synthesised by ATDBio Ltd (Southampton, UK) and were each labelled using two fluorescein dT monomers (Glen Research, Virginia, USA). Polymorphisms within codons 136, 154, 171 and 141 were investigated using the dual-labelled HyBeacons … how fast is a bugatti super sportWeb10 dec. 2010 · Six-colour HyBeacon probes for multiplex genetic analysis. Six-colour HyBeacon probes for multiplex genetic analysis Chembiochem. 2010 Dec … how fast is a brown hareWeb1 dec. 2001 · A novel probe technology, termed HyBeacons, is described, which provides a new homogeneous method for fluorescence-based sequence detection and allele … high end cycles