Chondrocyte rnaseq

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August undefined, 2024

WebJan 1, 2024 · Articular chondrocytes are largely postmitotic with their metabolism being stimulated by physiological magnitudes of mechanical load, inducing biosynthesis of molecules to preserve the tissue integrity and maintain a balance of catabolic and anabolic processes (Fig. 1) [11], [12], [13], [14]. WebChondrocytes ( / ˈkɒndrəsaɪt, - droʊ -/, [1] from Greek χόνδρος, chondros = cartilage + κύτος, kytos = cell) are the only cells found in healthy cartilage. They produce and maintain the cartilaginous matrix, which consists …

Microenvironmental mechanoactivation through Yap/Taz …

WebNov 17, 2015 · Tumour necrosis factor up-regulates protein kinase R (PKR)-activating protein (PACT) and increases phosphorylation of PKR and eukaryotic initiation factor 2- in articular chondrocytes. Biochem Soc... WebApr 11, 2024 · RNA-Seq Transcriptomic and Quantitative Proteomic Analyses. A total of 9727 transcripts were integrated from the clean data, ... The results collectively suggested a reduced chondrocyte differentiation of the Sr-treated group that supports the positive effect of Sr in the biological function of bone. gaspers rapport

Chondrocyte - Wikipedia

WebDesign: We performed RNA-sequencing on chondrocytes derived from LG/J (n = 16) and SM/J (n = 16) mice. We validated the expression of candidate genes and compared … WebThe existing bulk RNA sequencing (bulk RNA-seq) and single-cell sequencing (scRNA-seq) data for chondrocytes in the osteoarthritic knee joint provide the expression profiles of entire cell populations and individual cells, respectively. WebSep 19, 2024 · Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis Summary Background … gas pershing

Cells Free Full-Text Single-Cell RNA-Seq Reveals ... - MDPI

WebThe existing bulk RNA sequencing (bulk RNA-seq) and single-cell sequencing (scRNA-seq) data for chondrocytes in the osteoarthritic knee joint provide the expression profiles of … Web9:15 am. Keynote Presentation. “Surgery of the TMJ Past,Present and Future”. Peter Quinn, University of Pennsylvania School of Dental Medicine. 10:15 am. BREAK. TOTAL JOINT PROSTHETICS. 10:30 am. “Wear and Oxidation Analysis of Explanted TMJR Devices”. david haas you are mine lyricsWebRNA-seq was performed, comparing cells on glass to those on 5 and 55 kPa gels. Similar to both C3 treatment and siY/T, ... chondrocyte identity prior to therapeutic applications in restoring damaged or diseased articular cartilage. Materials and Methods: Cell culture: ATDC5 cells were cultured in a 1:1 Dulbecco’s modified Eagle’s medium ... gaspers kingdom city

"WebAug 3, 2024 · We replicated the association in RNA-sequencing (RNA-seq) data from maturing chondrocytes sampled at early and late time points during differentiation in vitro: we found that expression early in differentiation is significantly associated with p values from height GWASs ( p = 6.1 × 10 −10) and that this association remains after conditioning on … " - Chondrocyte rnaseq

Chondrocyte rnaseq

Combining segmental bulk- and single-cell RNA ... - ScienceDirect

WebApr 14, 2024 · This study analyzed the non-coding RNA and mRNA expression profiles obtained through next-generation RNA sequencing (RNA-seq) in the TIC of two pig breeds at 1 and 4 months. The functions and interactions between NKX3.2 and miRNA-326 were verified in chondrocyte models in vitro. WebJun 10, 2024 · This work expands our view of chondrocyte heterogeneity and rapid molecular changes in chondrocyte populations in response to joint trauma and …

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WebJan 14, 2024 · This study investigated the early genetic responses of healthy in situ chondrocytes under IL-1β attack with a focus on cell cycle and calcium signaling pathways. RNA sequencing analysis identified... WebAug 23, 2013 · Microarray studies provide insight into cartilage aging but do not reveal the full transcriptomic phenotype of chondrocytes such as small noncoding RNAs, pseudogenes, and microRNAs. RNA-Seq is a powerful technique for the interrogation of large numbers of transcripts including nonprotein coding RNAs.

Webchondrocyte proliferation over a 48h period when delivered at doses ranging between 1 and 10k ng/mL, resulting in cell count increases of 38-110% and 36- ... RNA-seq revealed that rhFGF18 protein analog and AAV2-hFGF18 treatment arms upregulated hyaline cartilage associated genes including COL2A1 and HAS2 (Fig 1), while downregulating ... WebChondrocyte culture is an efficient target system for evaluation of the pharmacological and toxicological effects of drugs acting on cartilaginous tissue. Many types of culture exist …

WebApr 9, 2024 · The quality of the 28 RNA-Seq data generated from blood, liver, and adrenal gland samples in the CT and H120 groups was reported by Dou et al. (2024) and showed an average of 9.46 Gb paired-end clean reads for each sample, with high quality (GC content: 50.81%, Q20: 95.06%, and Q30: 89.10%). WebConclusions: Novel chondrocyte subsets in nucleus pulposus were discovered through scRNA-Seq, which provided novel insight to understand the pathological change during the development of IVDD. Ferroptosis participated in disc degeneration pathogenesis and it might serve as a new target for intervening IVDD.

WebSep 3, 2024 · This article utilizes single-cell RNA-seq data from 22,338 human foreskin keratinocytes to reconstruct the transcriptional regulation of skin development and homeostasis genes, organizing them by differentiation stage and also into transcription factor (TF)–associated modules.

WebMay 12, 2024 · Severe and chronic low back pain is often associated with intervertebral disc (IVD) degeneration. While imposing a considerable socio-economic burden worldwide, IVD degeneration is also severely impacting on the quality of life of affected individuals. Cell-based regenerative medicine approaches have moved into clinical trials, yet IVD cell … david habib twitterWebChondrocytes are the cells responsible for cartilage formation (see Figure 25.1). In addition to the mechanical role of articular cartilage in the joints, cartilage is critical for … david haas you are mine chordsWebFeb 17, 2024 · Chondrocytes build cartilage in development, governed by the transcription factor SOX9. Using mouse models and transcriptome profiling approaches, we show here that SOX9 also has key roles to maintain growth plates open postnatally and to protect adult articular cartilage from osteoarthritic degradation. david habeeb attorney gaspers furlongWebJun 10, 2024 · Single-Cell RNA-Seq Reveals Transcriptomic Heterogeneity and Post-Traumatic Osteoarthritis-Associated Early Molecular Changes in Mouse Articular … gaspers losheimWebAug 1, 2016 · We have investigated using RNA-sequencing (RNA-seq) and systems analyses the changes that occur in damaged OA cartilage by comparing it with intact cartilage from the same joint. Methods Cartilage was obtained from eight OA patients undergoing total knee replacement. gaspers egg harbor township njWebApr 1, 2024 · RNA samples with an RNA integrity number >9.5 were used for RNA-seq. Assessment of cartilage specific genes in chondrocytes. We used real-time PCR to … gasper software