Bin2 phosphorylation mutant

Author: kgrf

August undefined, 2024

WebNov 14, 2024 · In mature MMCs or meristemoids, BIN2 is expressed, is upregulated and initiates BASL polarization redundantly with the MAPK module. The BIN2–POLAR–BASL … WebDa Som Lee1,2 iD Tae Hyeon An2,3 iD Hyunmi Kim2,3 iD Eunsun Jung4 iD Gyeonghun Kim5 iD Seung Yeon Oh6 iD Jun Seok Kim7 iD Hye Jin Chun8 iD Jaeeun Jung9 iD Eun-Woo Lee2,3 iD Baek-Soo Han3,10 iD Dai Hoon Han11 iD Yong-Ho Lee8,12 iD Tae-Su Han3,4 iD Keun Hur13 iD Chul-Ho Lee3,14 iD Dae-Soo Kim9 iD Won Kon Kim2,3 iD Jun …

Histone deacetylase HDA6 enhances brassinosteroid …

WebJul 1, 2024 · BIN2, a loss-of-function mutant of BIN2 and its two closest homologs, was hyposensitive to ABA in primary root inhibition, ABA-responsive gene expression, and phosphorylating ABA Response Element Binding Factor (ABF) 2 fragment by in-gel kinase assays, suggesting that these GSK3-like kinases function as positive regulators in ABA … WebDec 20, 2012 · Mutant MBP-mBZR1 protein containing bzr1–1Dmuta- tion alsophosphorylated GST-BIN2.Consistent fastermobility mBZR1-CFPproteins im-munoblot analysis transgenicplants, bzr1–1D mutation ap- peared vitro-phosphorylatedMBP-mBZR1 protein hadlittle effect unphosphorylatedBZR1 fusion protein (Fig. 2B), suggesting … green clean images

AT4G18710(BIN2) - Arabidopsis

WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced … WebDec 29, 2024 · BIN2 mutant expression assay The native promoter-driven genomic BIN2 gene (4058 kb) and the corresponding mutated genes were fused with the YFP tag and transferred into a Col-0 background. The … Webtion, BIN2 phosphorylation stabilizes MYBL2. Such dual role of ... in the mutant (Fig. S1A), as reported previously (45). We created double mutants of mybl2 with bri1-5, a weak loss-of-function allele of BR receptor BRI1 (47), and bes1-D, a gain-of-function mutant in the BR pathway (4). Although the single mutant mybl2 green cleaning chicago

Phytochromes A and B Mediate Light Stabilization of BIN2 to …

GSK3‐like kinase BIN2 phosphorylates RD26 to potentiate …

WebHere we show that the light-regulated PIF4 (phytochrome-interacting factor 4) factor is a phosphorylation target of the BR signaling kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2), which marks this transcriptional regulator for proteasome degradation. WebSep 6, 2009 · GST–BIN2 M115A is a BIN2 kinase-inactive mutant. (e) GST–BIN2 or GST–bin2-1 was first treated with BSU1–YFP immunoprecipitated from BR-treated (+ BL; 0.25 μM for 30 min) or untreated 35S ... flow process in performing the pcrWebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ... green cleaning burnham on sea

"WebBIN2 integrates multiple signaling pathways by phosphorylating a range of proteins including the components in light signaling. For example, PIF3, PIF4, and PIF5 can be phosphorylated by BIN2 and then degraded via the 26S proteasome pathway to control transcription and hypocotyl growth ( Bernardo-García et al., 2014; Ling et al., 2024 ). " - Bin2 phosphorylation mutant

Bin2 phosphorylation mutant

SnRK2s at the Crossroads of Growth and Stress Responses

WebAug 19, 2014 · Contrary to our expectations, BIN2 did not phosphorylate S75 or S77. Site-directed mutagenesis and subsequent in vitro kinase assays, employing single mutants (substitutions to A), revealed that... WebApr 20, 2024 · In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time.

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WebJan 30, 2024 · Importantly, the BIN2 phosphorylation of Thr280 residue restricts the function of CO in promoting flowering through affecting its DNA-binding activity. Moreover, we … WebThus, BIN2 is a good candidate to regulate PIF4 levels, as inactivation of this kinase by BL may result in the fastest-migrating form of this factor seen in BL-treated seedlings ( Fig. 1C ). A direct interaction of the full-length BIN2 protein and PIF4 was actually observed in yeast two-hybrid assays ( Fig. 2A ).

WebNov 1, 2002 · It was hypothesized that, in the absence of BR signals, BIN2 is a constitutively active kinase that would phosphorylate several positive BR-signaling proteins, rendering … WebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of …

WebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation and the phenotypes of transgenic plants … WebApr 11, 2024 · Highlights. As a major environmental factor impacting the seasonal growth and geographical distribution of plants, temperature change significantly affects crop quality and productivity. Ethylene is a gaseous hormone with an important role in plant growth, development, and multiple stress responses, including heat and cold. Temperature stress ...

WebBIN2 is a member of the GSK3-like kinase family, whose members can recognize substrates by either forming a stable protein complex with them or recognizing substrate epitopes that have been previously phosphorylated by an alternative protein kinase in a process known as priming phosphorylation.

WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes … green cleaning certification trainingWebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated … green cleaning chemical dispenser system flow process chart แบบฟอร์มWebApr 6, 2015 · AtSK21/BIN2 phosphorylation of ARF2 alleviates DNA binding and repressor activity of ARF2, ... Notably, mutant bin2-1 protein accumulates in greater quantities than wild-type AtSK21/BIN2 in plant cells (Peng et al., 2008). The BR biosynthetic inhibitor BRZ induces the accumulation of AtSK21/BIN2 in plant cells. flow process in thermodynamicsWebAug 13, 2024 · Phosphorylation mediated by protein kinases has been extensively studied, as it is one of the most important and ubiquitous posttranslational modifications. Protein kinase-mediated phosphorylation modulates many cellular processes, including the activities of enzymes, signal transduction, transcription, and metabolism. green cleaning columbia mdWebThe BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes … flow process of apparel manufacturingWebAug 25, 2016 · The regulatory mechanisms by which BRs and other signals regulate BIN2 activity and stability remain unclear. Although in BR signaling it is reported that BIN2 can be regulated by dephosphorylation of the Y200 residue (), the functions of many other phosphorylation sites remain unknown.In addition, overexpression of Y200 … flow process chart in method study